Friday, August 21, 2020

Open Homosexuality in America

Open Homosexuality in America Free Online Research Papers For my examination paper, I proposed to discover how the level of open gay people in the public arena has changed after some time in America. I might likewise want to know whether certain states in America depict radically various rates of gay people in contrast with each other. In our ever changing, and apparently regularly creating country, exactly how open and steady has American culture become in tolerating homosexuality? How is this mentality influencing the acknowledgment of homosexuality in gay people themselves, and how does the level of open gay people contrast with that of the past. I might want to explore this inquiry since homosexuality has been a steady contention. I accept that the concealment of homosexuality has been an issue from the beginning of time, and I might want to know whether the slowly developing help of homosexuality that is frequently depicted in the media is precisely mirroring a difference in values in American culture. Since forever, demonstrations of brutality and mistreatment against gay people have been predictable issues. I might want to know whether, in present day society, these components keep on beating gay people in their capacity to easily and apparently act naturally. While examining this theme, it was especially significant that I locate a general change in context in American culture with respect to homosexuality. In spite of the fact that examining the changing point of view was significant in this procedure, I additionally strived to discover solid rates and information that mirrored the apparently developing viewpoint. It was hard to locate an ongoing enumeration taken with respect to this data, so as my hunt advanced, I found that the general change in context turned into an increasingly critical piece of my examination. Through scholastic diaries, for example, the Journal of the History of Sexuality and The Gay and Lesbian Review Worldwide, I had the option to increase a lot of understanding into the subject of homosexuality in American culture. I found that after some time, the viewpoint of homosexuality has commonly mirrored the qualities forced upon American culture through the media (2). Homosexuality was generally dubious all through the late forties and fifties, when society was lowered in the possibility of â€Å"The American Dream†. This â€Å"American Dream† portrayed what the quest for bliss in American culture should comprise of. It focused on the significance of sexual orientation jobs and the ideal family; a couple in the suburbs with their youngsters and home-prepared meals consistently. The spouse was to watch out for the home professionally and the husband was to work a regular place of employment, (normally in an office or the like) five days every week. Homosexuality during this timeframe was once in a while known about and viewed as despicable. In the media, homosexuality was scarcely recognized, however when it was, the thought was immovably censured and viewed as hazardous. This depiction of homosexuality used dread as a technique for mistreatment (3). Men, ladies, and youngsters were informed that gay people are risky and compromising (2). It was not until the sexual upheaval during the sixties and seventies that homosexuality was given a recognizable nearness in America. Through crafted by Alfred Kinsley, the primary man to recognize and overview gay people in the public eye, the point of view of homosexuality started to continuously develop into the relative acknowledgment that is found in specific social orders in America today. While the point of view of homosexuality in America has changed after some time, the inquiry despite everything remains: has the measure of gay people in the populace changed, and provided that this is true, why? It appears just as the measure of gay people in the public arena has expanded in places where gay people feel good and are acknowledged (1). The distinction in gay populace between Kansas city and San Francisco, for instance, is radical. This at that point brings up the issue, are gay people increasingly acknowledged in specific zones since they move there and make the agreeable condition themselves, or on the grounds that the agreeable condition is now there set up. Would gay people become progressively acknowledged in specific zones additional time in the event that they forced their characters on specific social orders instead of escaping to ones that are increasingly steady and tolerating. The over all populace of gay people in American culture has reliably mirrored the qualities worried during explicit times. In spite of the fact that the acknowledgment of homosexuality in America has expanded altogether in specific states, there is an extraordinary contrast between the advances of those zones and the narrow minded dormancy of others. I accept, subsequent to experiencing the exploration procedure, that the measure of gay people in our populace has never really changed. Homosexuality has had a steady nearness since the beginning notwithstanding our evolving viewpoints. It appears as if the gay populace has never developed, however mirrored the estimations of every particular timespan. Like different minorities, homosexuality has been stifled, yet has never stopped to exist. While exploring homosexuality in America, I made some troublesome memories finding the solid numbers and rates of homosexual’s extra time. I discovered it was hard to look at what little rates I did discover, and that there were no genuine enlightening vacillations in them. While I battled with my exploration, I found that at the core of my examination subject was not exact numbers and figures, yet the over all adjustment in context of homosexuality in America. I was not astonished by my discoveries, however they empowered me to additionally see exactly what factors in the public arena influence the gay populace. V. 1. (Entryways, Gary J., Ost, Jason (2004). Getting Us Where We Live. Gay and Lesbian Review Worldwide. 19-21) 2. (Bennet, Lisa (2000). Fifty Years of Prejudice in the Media. Gay and Lesbian Review Worldwide. 18-27) 3. (Loftin, Craig M. (2007). Unsuitable Mannerisms, Gender Anxieties, Homosexual Activism, and Swish in the United States 1945-1965. Diary of Lesbian and Gay Studies. 578-596) Research Papers on Open Homosexuality in AmericaEffects of Television Violence on Children19 Century Society: A Deeply Divided EraInfluences of Socio-Economic Status of Married MalesRelationship between Media Coverage and Social andWhere Wild and West MeetResearch Process Part OneHip-Hop is ArtThe Effects of Illegal ImmigrationQuebec and CanadaComparison: Letter from Birmingham and Crito

Tuesday, July 14, 2020

E-Commerce - A Complete Guide

E-Commerce - A Complete Guide Electronic commerce or e-commerce is the process of buying and selling between businesses and consumers without a physical store, usually through an electronic medium. Often, this term refers to the internet as a marketplace but the term can also refer to retail activity through any electronic method. The technologies that support e-commerce include e-commerce or mobile commerce, electronic transfer of funds, internet marketing, electronic data interchange, inventory management systems, and online transaction processing among others. © Shutterstock.com | EllagrinIn this article, we will look at e-commerce basics such as, 1) what is e-commerce?, 2) making the e-commerce decision, 3) designing an e-store, 4) other e-store considerations, and the 5) pros and cons of e-commerce.WHAT IS E-COMMERCE? E-commerce can be successfully leveraged by businesses to create the sales aspect of their enterprise. It can be an effective market entry strategy for companies that do not have an established physical retail location.Categories of E-commerceThere are three categories of e-commerce depending on who is involved in the process. These are:B2B â€" Business to Business: When both the buyer and the seller are business entities, then their transaction is called business to business commerce. Entities such as manufacturers, traders, retailers come within this category.B2C â€" Business to Consumer: As in any traditional retail situation, a B2C model involves a business selling to an end user through an electronic medium.C2C â€" Co nsumer to Consumer: E-commerce has helped revived a version of the barter system where consumers are able to sell each other items that they own. Online auction sites and advertising portals are examples of this type of e-commerce.Types of E-commerce Activities Under the e-commerce umbrella there exist a variety of activities. These include:Online Shopping: A prevalent example of e-commerce is shopping via the internet. Virtual stores are created online, often boasting more variety of choices than are available at physical stores. There are also stores that exist only online, and benefit from the lack of investment into physical infrastructure.Electronic Payments: Payment systems for utility bills, phone, cable and internet bills, as well as online shopping are an important aspect of e-commerce. Here, security needs to be ensured regarding credit card and personal information.Virtual Auctions: One version of e-commerce is the online auction popularized by eBay. These websites offer customers the benefit of selling to others at the best possible price.Internet Banking: E-commerce has allowed many people to forego frequent visits to the bank and instead perform their regular banking activities online via secure banking services.Online Ticketi ng: Ticketing for almost all types of activities is now done online. This include travel ticketing such as air travel, bus and trains, as well as tickets for entertainment and sports events. This allows people to avoid queues and just go on the day of the event.Distribution ModesAs mentioned earlier, there are different ways in which e-commerce retailers offer their services. These can be defined as the pure-click, bricks-and-clicks and click-to-brick retailers.Pure-Click: These are retailers which only sell through an online location and have no physical storefronts.Bricks-and-Clicks: These are retailers that have both physical and virtual store locations.Click-to-Brick: These are retailers who began as online stores but eventually expanded to a physical location as a supplement to their online enterprise.What is E-Commerce? MAKING THE E-COMMERCE DECISIONBefore deciding to venture into an e-commerce business, it is a good idea to ask some basic questions to help establish readiness . Though this is not an exhaustive list but it does offer a good starting point for entrepreneurs.The Basic QuestionsIf you have an existing business, does it suffer from internet competition?Do customers ask for your website information?Do competitors or similar products have an online retail presence?The Logistics QuestionDo you have the resources to run a shipping operation?Do you have access to a storage and shipping area?If not, can it be acquired for a manageable cost?Will there be a need for additional staff to handle shipping?Creating a Web PresenceWill you use an existing platform to list products or create your own website?If using an existing platform, which model works best for you?If setting up your own platform, does the cost make business sense?Will you hire a professional to develop the website?ShippingWhat shipping mode will you use?Do the shipping costs and costs of shipping materials make business sense?Will you offer free shipping and absorb the cost?DESIGNING AN E-STOREThere are many benefits to creating an independent website that is not part of an existing platform. It allows the business owner more control over the mechanics of the shop as well as its appearance. There is better opportunities to create a unique brand presence.Develop Your Business Model or IdeaYou may be starting from the very beginning or trying to give an online presence to an existing. In any case, it becomes necessary to be clear on what will be sold, how it will be sold, who the customer will be and what the mechanics of the operation will be.Identify the product: Some products work well online as the decision to buy them can be made by viewing them online and using the information provided. Others may need to be examined in person before a transaction can happen. In any case, details such as shipping, inventory storage, product variety and the mechanics of creating the product to meet demand are some key areas to clarify.Identify a Niche: Even if you are clear abo ut the product you will sell and how it will be sold, it is important to be able to differentiate it from others in the market. The customer needs to have an incentive to choose your product from among the many others available in the market, both online and offline. Perhaps you can offer better product quality than the others. Or the product can offer a unique take on solving a problem. There may be special expertise than you can share or you can have a seamless buying experience.Test Market: It is a good idea to sell the product at a small store on an existing platform such as ETSY or ebay to gauge the level of enthusiasm and the overall response to the product. This will also help understand who is buying the product and how much are they willing to pay.Create a Sound Business Plan: With the first few steps complete, it is imperative to create a thorough business plan to ensure that all the steps are planned in detail. It will also help convince possible investors that you are pr epared and ready. Important elements should be the production costs, details of production, shipping costs and structure, taxes, labor costs, and e-store related costs.Acquire Website BasicsBefore a website can be designed, there is groundwork to be laid out. This includes decisions on the following areas:A Domain NameA Web HostA dedicated IP AddressA Private SSL CertificateA Shopping Cart ScriptA Payment Gateway (PayPal, Checkout etc)Setup WebsiteOnce the basics are setup, it is now time to develop the website and its customer interface. To begin, it is a good idea to finalize homepages as well as other static pages such as information about the company, contact pages and FAQ sections. For design, it may be a good idea to hire a web designer to ensure that the product is showcased on the best possible way and that the customers find it easy to navigate and order.Though it is often tempting to make a website too catchy, flashy and complicated, it is a better idea to keep it clean an d simple. The easier it is to navigate and find the right information, the higher the likelihood of a browsing customer turning into a paying one. There will also be more chances of repeat customers. The website should have cohesive fonts and pleasing colors and very few clicks necessary to get to checkout.It is also a good idea to have an option for customers to sign up for alerts and offers. This creates a link to the customers and allows the business to keep itself visible.Setup E-commerce Software and Merchant AccountThis step is necessary in order to allow customers to securely make purchases after viewing products online. The chosen software will save customer information, both personal and financial. In some cases, this same software can also be used to send emails to customers. Whichever software is chosen will impact user experience so it is a good idea to carefully research this step.There will also need to be an account setup with a bank to allow customers to use credit c ards. Banks are often an expensive option and a less pricey alternative is a payment gateway such as PayPal.Setup a Maintenance ScheduleIt is necessary to monitor the online shop and its reports to keep an eye on how things are going. There may be a need to update software at the backend to keep up with improvements as well as continue to improve user experience and fix bugs.Building an E-Commerce Business Startups Uncensored 11 OTHER IMPORTANT E-STORE CONSIDERATIONSDoes Your Website need a Blog?Often, websites are accompanied by a blog. Blogs should only be undertaken however, if there is enough time to update it regularly with material of interest and relevance. There are benefits to such an arrangement including:A Channel of Communication: A blog will allow a place where positive conversation can be initiated with current and potential clients. It allows an insight into consumer thought and trends.Credibility: A blog can allow an entrepreneur a platform to showcase their knowled ge and expertise and therefore create credibility.A Sense of Community: Interaction through a blog can create a sense of community within customers which is something people cherish.Human Element: Often, people respond better to brands when they can place a face alongside. Blogging allows a personal repertoire to be established and a personal brand narrative to be created.Better Search Engine Ranking: Blogs have a better chance of showing up during web searches than plain e-commerce sites. The original content in a blog is more likely to be picked up than similar supplier data that is usually found on e-stores.Search Engine Optimization © Flickr | Paloma GómezStrategies and tactics to increase website traffic are known as search engine optimization or SEO. SEO helps a website get placed higher in a search engine’s results page. SEO helps a website become searchable by using both technical tasks to allow search engines to find and index the site for the right keywords and marketing tasks to make the site appealing.Monitor Rankings: Use tools to keep an eye on your current rankings.Keywords: Appropriate keywords should be used in all areas of the website including headings, content, website address, and images. In fact, describing images are a great SEO tactic. Keywords should be considered search terms but be wary of putting in too many of them. This may flag you as a spammer and the website could be ignored for keyword-stuffing.Link Back to Yourself: Linking back to own pages or archives help SEO activities as the more pages that point to a page, the higher the chances of it showing up in initial search results. Sitemaps: Sitemaps that list all the content inside a website make it easy for search engines to scan and index the website, this increasing chances of search result success.Relevant URLs: If the right keywords appear in the URLs, it makes the website more search engine friendly.Avoid Flash: Although Flash looks good, it does not add to search engine friendliness.Describe Images: Images cannot be searched. So if there are words in an image or it is relevant to keywords, it will not be seen by search engines. A good idea to fix this problem is to add image descriptions, and try to use relevant words in them.Keep Content Fresh: The more updated the content is the more chances of it pulling in customers. Website blogs can help with this aspect.Social Media: If links to content on the website are shared repeatedly on different social media outlets, it works towards increasing search engine results and customer views.Link to Others: By developing relationships with relevant websites and blogs, you can link to them thereby creating more visibility. This should be done with relevant sites which enjoy good reputations otherwise this tactic may end up doing more harm than good.E-COMMERCE PROS AND CONSThere are some clear advantages and disadvantages of using e-commerce to sell your product. There are also some less clear ones that can serve as both an advantage and disadvantage. These pros and cons are listed below.Advantages of E-commerceThere is no need to wait in lines.There is ease of access to stores that may be further away or even overseas. This can be especially useful for consumers in a rural locations.There is no need to set up and invest in a physical store.There are many options available, both in terms of competitors and variety of products offered by each of them.There is no store timing restrictions and customers can shop 24 hours a day.Internet commerce has allowed customers various platforms to sell to each other which is a valuable benefit.In the case of digital items, the purchase is instant and there is no need to wait for delivery.There is unlimited space to sell as many items as a storage locations can hold. This means more variety than a physical store location.Disadvantages of E-commerceThere needs to be relatively fast and reliable internet service.There also need to be a device that can allow access to the store such as a smartphone or a laptop.The shopping experience can be somewhat impersonal and clinical.There is no way to experience a product before purchase. The luxury of touching and feeling the item is taken away.There is the ever present threat of credit card theft and fraud.In the case of physical goods, the customer has to wait to receive their purchased items.This is often confusion in terms of regulations as taxation as the buyer and seller may be in different locations that have different taxation mechanisms.Both Advantage and DisadvantageEasily available coupons and deals are both a pro and a con as this be comes a pro and an expectation by the customer and it draws them back again. On the other hand, it is a con because this comes at a cost to the seller.Easy price comparisons are a pro and a con because this can either draw a customer to the business or filter the business out in a price based search.In today’s world, there is usually no choice for a business when it comes to online presence. With demanding customers and increased competition, online stores allow businesses to reach new customers in faraway locations and in many cases save costs on physical storefronts.The Future Of Ecommerce | Ask A VC Image credit:  Flickr | Paloma Gómez under Attribution-ShareAlike 2.0 Generic.

Thursday, May 21, 2020

World history The change in Imperialism Essay - 658 Words

World history: The change in Imperialism Social, economic, and political change altered the nature of imperialism in three principal ways. One was the amplified need for natural resources, two was the new understanding of economics, and three was the expanding sense of what it meant to be civilized. All of these events and ideas were lead up to by the industrial revolution. The industrial revolution altered the course of history in numerous ways but the most significant is certainly that of imperialism. The industrialization created a rise in population and thus a rise in demand. This in it of itself did not lead to the need for conquest but it was certainly a contributing factor. The Industrial revolution began to produce goods†¦show more content†¦Now what is civilization, well according to the Europeans it was white, privileged, European men and women. So this new idea plays into slavery. People would go on trips to â€Å"civilize† and enslave the people that they met. They would strip them of original culture and procreate their lands and resources. And coincidentally this worked right be side the Europeans need for resources, because these â€Å"mission trips† weren’t about mission at all they were about conquest and finding new territories and peoples that they could use to their benefit. â€Å"Everyone who knows little about aboriginal races is aware that these races which are low type mentally and who at the same time are weak in constitution rapidly die out when their country becomes occupied by a different race much more rigors and robust pushing themselves.† This idea stated by George Pullwiok was the general idea of most people in this time period. So how did this contribute to the conquering of people? Well this thinking also created the idea of the white means burden. Europeans commonly believed that it was their duty to civilize those who they saw to be inferior, so it was yet again another excuse for them to go and attack, conquer, and colonize countries. The downfall of states that did not industrialize was clearly apart of the change in imperialism because allowed other superior states to gain control and to try and dominate. Countries likeShow MoreRelatedEssay On Ecological Imperialism1618 Words   |  7 PagesEcological Imperialism and the New World Starting from the times that countries started travelling overseas and conquering other lands, they used this newfound power to supply their own greedy desires through ecological imperialism. This process altered this new landscape and homogenized it into the ways Europeans wanted it with the natives taking all or most of the side effects, while all the benefits went to the colonizers. Using various sources from authors such as Crosby, Piper, SandlosRead MoreBrionna Johnson. Mr. T.Kemiksizgil, Period 4. Mrs. K. Prinzo,1310 Words   |  6 Pages Period 4 Mrs. K. Prinzo, Period 6 03 March 2017 European Imperialism Good for Colonized Countries? Imagine you re in the 1700s and live in South Africa or another colonized country. You’ve gotten used to the new lifestyle, but then the Europeans come in and force imperialism onto you and your country. Being obliged to do something is what happened in South Africa. South Africa got colonized by Britain in 1795. Imperialism spread in the 1900s to Africa. So Africa was forced into havingRead MoreChange and Continuity738 Words   |  3 PagesDeborah Heckwolf A.P. World History – 8th period Triangular trade and Erie Canal also started during this period creating a bigger market to produce. Trade between nations on a global scale has changed through our history dramatically with transportation. 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European imperialism boosted Europe’sRead MoreWomen’s Role During the Inca Empire961 Words   |  4 Pages(1438–1533) and after Independence ABSTRACT In the following paper, we will see the role of women change from a submissive role during the Inca Empire to an activist in the present. Many empires developed and declined before 1500 CE. Imperialism had great impact on the status and roles of women, for example in noble women’s roles in marriage, religious rituals, power structures and legal rights (â€Å"Imperialism and Colonialism† web). In the following paragraphs, we will understand women’s role in the IncaRead MoreImperialism in the 19th century1746 Words   |  7 Pagesgreat deal of Imperialism in the 19th century, led by mostly westerners from Europe. Imperialism is the act in which one nation extends its rule over another. 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American imperialism plays in important role through history as the cause of not only the genocides of the Native Americans, but theRead MoreGlobalization: Western Imperialism by Another Name Essay934 Words   |  4 Pagessituation for many indigenous people across the world from as far back as the 13th century. Throughout history dominant nations have been advancing their own civilizations through exploiting the land, labour, raw materials and markets of weaker nations. This process of capitalist engulfment is known as imperialism. But is this the same as globalisation? There are many similarities between globalisation and imperialism. 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During this time imperialism was very prevalent and the events that took place during this time had lasting effects and still affect us to this day. While imperialism could be seen as beneficial, especially to the

Wednesday, May 6, 2020

Macbeth Is the Most Interesting Character in the Play Macbeth

â€Å"The most interesting character in Macbeth is Macbeth himself.† Do you agree? Why? Macbeth is a complex and well developed character, while there are several interesting characters in the play, Macbeth is by far the most interesting. He is a tragic hero who experiences a slow and painful mental decline which is expressed in such a way that the audience is mesmerised. The most interesting aspect of Macbeth’s character is that it is flawed. The audience can identify with his human frailties. Despite the fact that Macbeth commits evil and is drawn to the successes he thinks evil can provide him, one can tell he has goodness in him. Shakespeare reveals Macbeth’s moral development through the use of symbolism in the soliloquys which portray†¦show more content†¦He cannot even bear to look back upon it; â€Å"I am afraid to think what I have done; Look ont again I dare not.† This gives us an understanding of how he is only human and interestingly gives the audience a sense of sympathy. Macbeth’s mental and moral deterioration throughout the play engages the audience illustrating how guilt overwhelms his conscience He believes he hears voices crying â€Å"Macbeth has murdered sleep† this demonstrates how he is battling against his morals and his ambition. His good qualities are battling his bad thoughts and this is the main reason for his mental downfall which makes for a deeply engaging plot. Macbeth goes from being a man of bravery, strength, honour yet he slowly loses these qualities. He once believed that killing a good man was an evil, un-worthy thing to do yet by the end of the play he is killing the people he once had close relationships with to get himself out of the mess that was dragging him deeper into despair and tragedy. This process is enthralling for the audience who cannot resist watching him go to any length to save himself as his morals go into deep decline. Ambition has completely taken over him in the soliloquy in which he states; à ¢â‚¬Å"I have no spur to prick the sides of my intent, but only Vaulting ambition, which o’erleap itself and falls on the other.† In this instance Macbeth is interesting because he realises that the only thing that is making him want to kill Duncan isShow MoreRelatedLady Macbeth1003 Words   |  5 PagesLady Macbeth Lady Macbeth is the most interesting and complex character in the play. She is, in fact, the point on which the action pivots: without her there is no play. Macbeth s most interesting and complex character is most certainly, as the question states, Lady Macbeth. The purpose of this essay is to describe Lady Macbeth s role in the play and discuss why this makes her the most fascinating character. 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In Act one, Scene one, line 12, the witches say, â€Å"Fair is foul and foul is fair.† This is interesting as they are suggesting good and evil as beingRead MoreWilliam Shakespeare s Macbeth 1318 Words   |  6 PagesShakespeare s tragedy, Macbeth, follows a man’s bloody rise to power, which hinges on murder of the Scottish king named Duncan. In my opinion, the play s most memorable character is the wife of this man, Lady Macbeth. Similar to her spouse, Lady Macbeth s lust for power plunges her into madness. So much so that Lady Macbeth turns to witchcraft transforms herself into an desexualized evil spirit to quench her thirst to become a man. The play is filled with moments when characters reveal vital information

Unknown Lab Report Free Essays

Unknown Lab Report #1 Unknown #1 April 25, 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result, various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. We will write a custom essay sample on Unknown Lab Report or any similar topic only for you Order Now By identify the microbe, the safety of the water will be known to those around it. Materials and Methods Unknown #1 was found in a nearby pond that was created by an earthquake. Some of the various methods introduced and practiced in class were applied in identifying the microbe. Procedures were followed as stated in the index of the Virtual Unknown Software. First the shape and color of the microbe was noted. This is done to know the morphology of the microbe. After concluding that the microbe was Gram negative rods, it was inoculated into a previously prepared test tube. Then various tests were performed to isolate and identify the unknown microbe. Table 1 shows the tests, purpose, reagents and results of the various tests performed. Each test was performed according to the index of the Virtual Unknown software and the following tests were performed: 1) Adonitol Fermentation 2) Cellobiose Fermentation 3) Maltose Fermentation 4) Lactose Fermentation 5) Raffinose Fermentation 6) Sorbitol Fermentation 7) Sucrose Fermentation 8) Melibiose Fermentation 9) Indole Production Results Table 1: Biochemical Tests Results Test| Purpose| Reagents| Observations| Results| Adonitol Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) adonitol as a carbon source. | None| Turned pink| Negative adonitol test| Cellobiose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) cellobiose as a carbon source. | None| Turned Yellow| Positive cellobiose fermenter| Maltose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) maltose as a carbon source. None| Turned Yellow| Positive maltose fermenter| Lactose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) lactose as a carbon source. | None| Turned Pink| Negative lactose fermenter| Raffinose Fermentation| To determine whether the microbe can use sugar raffinose for carbon and energy. | None| Turned pink| Negative raffinose fermenter| Sorbitol Fermentation| To determine if the microbe can ferment the carbohydrate sorbitol as a c arbon source. None| Turned yellow| Positive sorbitol fermentation| Sucrose Fermentation| To determine if the microbe can ferment the carbohydrate sucrose as a carbon source| None| Turned Pink| Negative sucrose fermenter| Melibiose Fermentation| To determine if the microbe can ferment the carbohydrate (sugar) melibiose as a carbon. | None| Turned Pink| Negative melibiose fermenter| Indole Production| To determine whether the microbe can produce indole from the amino acid tryptophan. | Add five to ten drops of Kovac’s reagent to the test tube. The reagent does not mix with water and forms a thin layer above the broth. The reagent reacts with idole to produce a cherry red ring. | Red ring at the top of broth| Positive indole test| Flowchart Unknown#1 Gram Stain Gram Negative Rod Adonitol Test (-) Positive Negative Klebsiella ornithinolyticaCitrobacter koseri Klebsiella oxytocaEnterobacteraergenes Klebsiella oxytocaEscherichia fergusonii Klebieslla pneumoniae sspKlebsiella ornithinolycia pneumoniaKlebsiella pneumonia Klebsiella oxytocassp ozaenae Klebsiella terrigenaKlebsiella pneumonia ssp Leclercia adecarboxlatarhinosclermatis Klebsiella terrigena Leclercia adecarboxylata Mollerlla wisconsinisis Providencia alcalifaciens Providencia rettgeri Serratia fonticola Serraria rubidea Cellobiose fermentation (+) Positive Negative Buttiauxella agrestis Edwardsiella hoshinae Enterbacter cloacae Edwardsiella ictaluri Enterbacter intermedius Edwardsiella tarda Enterbackter sakazakii Edwarsiella tarda Kluyvera ascorbata biogroup 1 Rahenlla aquatilis Escherichia blattae Escherichia coli Ewingella Americana Morganella morganii ssp morganii Morganella morganii ssp sibonii Proteus mirabilis Proteus myxofaciens Proteus penneri Proteus vulgaris Providencia rustigaianii Providencia stuartii Salmonella bongori Salmonella cholerasuis ssp arixonae Salmonella paratyphi A Salmonella typhi Serratia marcescens Serratia proteamaculans Shingella prtoeamaculans Shingella dysnteriae Shingella flexneri Shingella sonnei Tatumella ptyseos Yersinia pestis Yersina pseudotubercilosis Maltose Fermentation (+) PositiveNegative NoneNone Lactose Fermentation (-) Positive Negative Buttiauxella agresitsCitrobacter amalonaticus bigroup 1 Enterobacter cloacae Enterobacter amnigenus bigroup 1 Enterbacter intermedius Enterbacter vulneris Enternbacter sakazakii Serratia odonifera bigroup 1 Kluyvera ascorbata Serratia plymuthica Rahnella aquatillis Raffinose Fermination (-) PositiveNegative Citovacter amalonaticus biogroup 1Cedecea davisae Enterbacter amnigenus biogroup 1Cedecea lapagei Enterobacter gergoviaeEnterbacter cancerogenus Escherichia vulnerisEscherichia hemannii Serratia odorifera biogroup 1Hafrina alvei Serratia plymuthicaYersinia kristenernii Sorbitol fermentation (+) PositiveNegative Cedecea neteriCedecea davisae Serratia ficaria Cedeca lapagei Yersinia enterocolitca Enterobacter cancerogenus Yersinia frederiksenii Escherichia hermannii Hafnia alvei Yersinia kristensenii Sucrose fermentation (-) PositiveNegative Cedcea neteriEnterbacter amnigenus biogroup 2 Serratia ficariaSalmonella choerasuis ssp hotenae Yersinia enteroclitica Yersinia frederiksenii Melibiose Fermentation (-) PositiveNegative Enterbacter amnigenus biogroup 2Citrobacter freudii Salmonella cholerasuis ssp houtenaeCitrobacter amalonaticus Indole production (+) PositiveNegative Citrobacter amalonaticusCitrobacter freundii Discussion/Conclusion After conducting a variety of various tests, it was concluded that Unknown #1 was Citrobacter amalonaticus. Once the morphology of the microbe was identified, it was isolated and inoculated into a test tube. The nine tests performed on the microbe were done according to the index of the virtual unknown software and the results of each test correlated to the results anticipated. As a result, it was concluded that the unknown #1 microbe was Citrobacter amalonaticus. Citrobacter amalonaticus has a gram negative rod-shape. They can be found in the digestive system and are linked with digestive disorders such as diarrhea. They can also be present in the urinary tract and most commonly causes Urinary Tract Infections. Citrobacter amalonaticus is also known as an opportunist pathogen. Opportunist pathogens are pathogens that do not cause much damage to healthy individuals; however, it can cause very severe damage to those with a weakened immune system. Besides being found in the intestines of humans, Citrobacter amalonaticus is usually found in the ground (soil), in the air and in the intestines of animals such as bats. (1) Citrobacter amalonaticus also has some positive effects. It has the ability to recycle hydrogen. In addition it is capable of breaking down nitrates into nitrites in the nitrogen cycle. This process is commonly used in the biodegration industry. There tannic acid degrades into tanneries. (2) Citrobacter amalonaticus is also capable of combing metals and phosphates. This allows for the harmful substances such as uranium to be removed from water, soil and uranyl phosphate crystals. (2) References: Citrobacter amalonaticus A cousin to Citrobacter Freundii. http://www. citrobacterfreundii. org/citrobacter-amalonaticus/citrobacter-amalo How to cite Unknown Lab Report, Essay examples Unknown Lab Report Free Essays Background: In Jane Horack’s article â€Å"Staphylococcus epidermidis†, S. epidermidis is described as â€Å"gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages. † The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. We will write a custom essay sample on Unknown Lab Report or any similar topic only for you Order Now When viewed under a microscope S. epidermidis will appear in chains, pairs, or grape-like clusters (Horak 1). Taxonomically, the species S. epidermidis falls in the genus Staphylococcus, which is in the bacterial family Staphylococcaceae. S. pidermidis is in the phylum Firmicutes, under the Bacillales order. Like many members of the genus Staphylococcus, S. epidermidis is non-motile, as well as non-spore forming (Horak 1). The species is also facultative anaerobes, but not all strains of S. epidermidis will ferment. S. epidermidis is catalase positive, and this sets them apart from other gram-positive cocci, such as Streptococcus. They are also urease positive, cannot utilize Mannitol, and are resistant to several antibiotics (Horak). Staphylococcus epidermidis is considered â€Å"an opportunistic pathogen. It usually has a symbiotic relationship with its host, and for this reason it rarely causes diseases and is usually considered nonpathogenic (Avdic, Habes, and Avdic 3885). Rece ntly though, the microorganism is becoming the common cause of nosocomial infections. In â€Å"Microbiology: with diseases by taxonomy†, Richard Bauman defines a nosocomial infection as â€Å"a disease acquired in a healthcare setting. † These infections are often found with implants and plastic items that have inserted into the body, such as catheters, pacemakers, and urinary catheters (Avdic, et. l 3885). â€Å"The ability of this microorganism to causes infections is primarily due to its ability to form biofilms on synthetic surfaces of implanted medical devices† (Avdic, et. al 3885). Biofilms are considered the primary residence of microorganisms in nature, and are composed of numerous microorganisms (Bauman 173). Numerous studies clearly show that a large number of hospital strains have the ability to form multilayered biofilms on inert surfaces (Avdic, et. al 3886). The formation of biofilms has serious clinical consequences and is the cause of many persistent and chronic infections particularly in patients who have long been hospitalized or are in critical condition. (Avdic, et. al 3886). These infections are more severe in patients with compromised immune systems, such as the elderly, young children, or cancer patients (Horak 2). One of the biggest concerns with S. epidermidis is the frequency of these nosocomial infections. As these infections become more prevalent, antibiotic resistance is quickly increasing (Horak). In Michael Otto’s article â€Å"Staphylococcus epidermidis – the â€Å"accidental† pathogen†, he suggests the reason for this resistance is due to antibiotic overuse. Vaccination and decolonization has been discussed as preventative measures, but there is currently no vaccine for S. epidermidis, and the effect of decolonization may prove counterproductive. If S. epidermidis was removed from the normal human flora, it might allow other microorganisms to colonize (Otto). For those reasons, it is commonly agreed that best way to deal with S. epidermidis infections is by a series of preventative measures. Staphylococcus Epidermidis biofilm Staphylococcus Epidermidis biofilm Materials and Methods: There were series of tests performed before the unknown could be properly identified. The unknown was first plated using the antiseptic technique. The aim of the antiseptic technique is to separate colonies of each microorganism so it may become easily distinguished and pure colonies will isolated. The process began by flaming the inoculating loop over the Bunsen burner to kill any pre-existing bacteria. The neck of the vial containing cells of the unknown was also flamed to kill any existing microorganism. The inoculation loop was then dipped in the nutrient broth, and neck of the vial was flamed once again. After a brief cool-down, the inoculating loop was streaked across a nutrient agar plate using the Quadrant Streak method. The Quadrant Steak method involves smearing the bacteria in quadrant one. The quadrant begins at the top of the agar plate, the loop is smeared into a downwards motion. Following this process, the inoculating loop was flamed once again. After the loop cooled down, a single colony from quadrant one was targeted and smeared into quadrant two, and was followed by flaming the inoculating loop. The process was repeated to smear bacteria into the remaining quadrants three and four. The agar plate was then turned upside to prevent condensation from dipping onto the culture and causing contamination. The process was repeated four additional times on to a phenylethyl alcohol agar (PEA), a Mannitol slat agar (MSA), a MacConkey agar (MAC), and an eosin methylene blue agar (EMB). Following the incubation of the several agar plates, the growth of colonies allowed for the next step in diagnosis – staining. The simple stain was performed first and can be used to determine cell shape, size, and arrangement. The process required the inoculating loop to be flamed once more, and a loopful of the culture was smeared onto a clean slide. A drop was water was applied onto the slide, and mixed to create a thin film. After the water had dried, the slide was heat-fixed. The heat-fixture ensured the bacteria would not be removed during the staining process. The slide was then covered with the agent Methylene blue for thirty seconds, and the excess dye was removed with water the gentle blotting. S. epidermidis – simple stain S. epidermidis – simple stain Following the simple stain, the Gram Stain was performed. The Gram Stain is a procedure used to determine the presence or lack of peptidoglycan in the cell walls of a bacterium. The process began with flaming the inoculation loop, and smearing a colony of bacteria onto a clean slide. A drop of water was applied and smeared to create a thin film. After the water had completely dried, the slide was then passed through the Bunsen burner. This adhered the bacteria to slide. The slide was then stained with Crystal Violet for sixty seconds, and completely rinsed with distilled water. The slide was rinsed with distilled water after every step. After the crystal violet, the slide was covered in Iodine for sixty seconds, and rinsed. Following the Iodine, the slide was decolorized with 95% ethyl alcohol-acetone for three to five seconds, and rinsed. The last step was the counterstain with Safrain for thirty seconds, rinse, and gently blot off the excess water. If the bacteria retained a purple stain, it was considered Gram-positive. However, if the bacteria possessed a pink or red stain, it was considered Gram-negative. S. epidermidis Gram-stain S. epidermidis Gram-stain How to cite Unknown Lab Report, Papers

Thursday, April 23, 2020

Job Shadowing Experience free essay sample

â€Å"How would you feel about taking out IVs from a dead man?† asked Nurse Sue. My instinct was to run. Should I really be scared? It shouldn’t be too bad. This could actually be pretty cool. I queasily walked into the room. Approaching the body, I touched his cold, harden leg. And then, my fear was replaced with compassion for what he and his family had endured. I took out the IVs as instructed by the nurse. This process filled me with accomplishment, knowing I contributed to his care, feeling proud of myself because I thought I couldn’t handle it. The day ended and Nurse Sue was sad to see me go. She told me, â€Å"Sarah, it was a pleasure working with you. You were excellent around the patients and I know you will be a great nurse someday.† This made me think. I never thought I would be this excited to grow up. We will write a custom essay sample on Job Shadowing Experience or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page I can’t wait for the day that I become†¦a nurse. Leaving the hospital that day I knew nursing was for me. I felt confident after finishing taking care of the dead man. It made me realize that if I could handle touching someone that wasn’t alive, I will have more courage in other uncomfortable situations.